Time-Resolved Infrared Spectroscopy of RNA Folding
نویسندگان
چکیده
منابع مشابه
Time-resolved infrared spectroscopy of RNA folding.
We introduce time-resolved infrared spectroscopy as a powerful method to study the kinetics of RNA folding and unfolding transitions. A laser-induced temperature jump is used to initiate a perturbation in the RNA structure. A probe laser, tuned to a specific infrared absorption of the RNA, is then used to monitor the subsequent relaxation kinetics. A 10-ns pump pulse permits the investigation o...
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The timescales of protein folding events range over many orders of magnitude. In order to understand the complex folding mechanisms, peptides with well-defined secondary structure are often used as model systems as they may be regarded as smallest folding units of proteins. The formation of secondary structure elements occur on the nanosecond to low microsecond time scale. Thus, stopped-flow te...
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The development of fast time-resolved mid-infrared (MIR) spectroscopy since the 1980s has provided an effective tool for the study of transient chemical species in dynamic systems.1,2 Interest in this particular spectroscopy stems from the virtual universality of infrared (IR) absorption, the rich molecular structural information contained in the MIR spectrum, and the fast time scale of vibrati...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2005
ISSN: 0006-3495
DOI: 10.1529/biophysj.105.061531